Our results further validate the role of prestin as a biomarker for SNHL in the chronic setting. We also highlight that the greatest value of this novel biomarker is within the range of hearing loss, which may be primarily attributed to OHC disruption and loss. In more severe hearing loss, the likely involvement of other cellular mediators (e.g., inner hair cells, spiral ganglion cells, and stria vascularis) diminishes the role of prestin as a biomarker. Here, SLC26A5 is linked to hearing loss disorder.