- MUC16-BiTE mediated T cell activation and target cancer cells specially.- OAd-MUC16-BiTE–mediated enhanced T-cell–mediated tumor cell killing and bystander effect.- OAd-MUC16-BiTE enhanced infiltration of CTLs and reversed the immunosuppressive TME.- Promoted T cell trafficking to the tumor by increasing pro-inflammatory factors and decreasing anti-inflammatory factors.- Local administration lowered toxicity and systemic exposure. This evidence concerns the gene MUC16 and cancer.