Indeed, it has been observed that loss of function associated with PTEN can impact the secretome of glioma and other cancer cells, including chemokines and cytokines that impact tumor growth, metastasis and even the intravasation and reprogramming of lymphoid and myeloid cells.27–31 These findings thus prompted the question of whether the loss of PTEN and/or hyperactive PI3K signaling might also affect the biogenesis and/or cargo of extracellular vesicles (EVs), which represent another major component of the cancer cell secretome. The gene discussed is PTEN; the disease is glioma.