We next performed validation assays in the UM-SCC-49 cell line model.<h4>Results</h4>Our data prioritized 207 genes as pivotal for cisplatin resistance in HNSCC, including novel genes <i>VGLL3, CIRHA1, NCOR1, SPANXA1, MAP2K7, ULK1,</i> and <i>CDK16</i>. This evidence concerns the gene SPANXA1 and head and neck squamous cell carcinoma.