The results of small animal live imaging system chemiluminescence showed that the fluorescence signal was substantially decreased in the abdominal area of the nude mouse model after the TOX3 knockout (Figure 3F), and H&E staining of liver in situ tumor sections showed that the number of hepatic in situ tumors was also decreased after TOX3 knockout (Figure 3G). The gene discussed is TOX3; the disease is neoplasm.