The results showed that the luciferase activity for HIF1A decreased to 47.98 ± 2.24%, 42  ±  1.15%, and 44.89 ± 0.62%, respectively (Fig. 2F) as compared with control (P < 0.001) The results confirmed the MYC, ZEB1, and HIF1A genes as candidate targets for miR-561-3p, and also these 3′-UTRs as functional target sites for this microRNA in BC cells. The gene discussed is ZEB1; the disease is breast cancer.