To identify whether Mi-2β depletion induced an immune response in B16F10 melanoma cells, mouse graft melanomas with shMi-2β virus-infected B16F10 cells were treated using anti-PD-1 antibodies (10 mg/kg) at day 6, 9, 12, 15 and 18 after tumor cell inoculation in immunocompetent C57BL/6 mice. This evidence concerns the gene CHD4 and neoplasm.