We next tested the effects of cancer-associated PBRM1-BD4 missense variants on full-length PBRM1 histone Kac binding by incubating transduced Caki-2 nuclear lysates with biotin-labeled H3K14ac and H3K14/18/23/27ac peptides bound to streptavidin resin. This evidence concerns the gene PBRM1 and cancer.