In this study, we identified a novel GC-related circRNA termed circGLIS3 from exon 2 of the GLIS3 gene with a circBase ID of hsa_circ_0002874, which was upregulated in GC tissues, and promoted tumorigenesis and carcinogenic progression by regulating the miR-1343-3p/PGK1 pathway and inhibiting vimentin phosphorylation at Ser83. Here, GLIS3 is linked to gastric cancer.