After mitochondrial inhibition, we also found that Tig/Tet treatment significantly inhibited the proliferative pathways (lower pAKT and cMYC) and downregulated the metastatic signaling (reduced SRC and FAK phosphorylation) in high-metastatic cells. In contrast, significant cleavage of caspase-3 and PARP and activation of autophagy, were observed, indicating the dual mode of cell death by these antibiotics in high-metastatic CRC cells. Here, PTK2 is linked to colorectal carcinoma.