Three AML cell lines of diverse cytogenetic origin, THP‐1 (MLL‐AF9, TP53mut), HL‐60 (amplified MYC, TP53del) and MV4‐11 (FLT3ITD, MLL‐AF4), lost colony‐forming capacity after transduction with shRNA targeting NFATC2 (shNFATC2‐1 and shNFATC2‐2), compared to an NTC shRNA (Fig. 2A; P < 0.05). The gene discussed is AFF1; the disease is acute myeloid leukemia.