In the final experiment, to investigate whether this method can comprehensively elucidate the dynamic modulations of sphingolipids, we employed this method to measure sphingolipid levels in mouse colon cancer cell lysates with enhanced expression of S1P lyase (SPL) or its inhibition using the CRISPR/Cas9 system, as previously described27. Here, SGPL1 is linked to malignant colon neoplasm.