While this work demonstrates that selective removal of miR-33 is sufficient to elevate AgRP neuronal activity, increase food consumption, and promote obesity and metabolic dysfunction in both male and female mice fed a high fat diet, this study did not determine whether the more modest difference in body weight and metabolic function that occurred with age in chow diet fed mice would also be observed in miR-33AgRPiKO mice. This evidence concerns the gene AGRP and obesity due to melanocortin 4 receptor deficiency.