We profiled the whole transcriptome of macrophages, T cells, and B cells using the GeoMx® DSP WTA assay (Fig. 1A), by selective collection of UV-cleavable probes from distinct masks generated by immunofluorescent staining of the morphology markers CD68, CD3 and CD20 (pertaining to macrophages, T-cells and B-cells, respectively), in RLTs (n = 24) and DLBCL patients (n = 64) for a total of 702 areas of interest (AOIs) (Supplementary Fig. 1A, representative images in Fig. 1B, C and Supplementary Fig. 1B–D). Here, CD68 is linked to diffuse large B-cell lymphoma.