C5AR1 and neoplasm: Multi-parametric flow cytometry of cells dissociated from paraspinal Nf1fl/fl;DhhCre PNF (Wu et al, 2008) or normal nerve/Dorsal root ganglia (DRG) (WT) showed that C5aR1 is expressed on nearly 40% of macrophages and 5–10% of dendritic cells in WT and PNF (of live CD45+ cells), and that the number of C5aR1+ cells increases in tumor largely because of the known increased numbers of immune cells in tumors and not an increase in C5aR1 protein on macrophages (Fig 1D–F).