In this study, we integrated computationalmodeling-based rationaldesign together with directed evolution utilizing yeast display andhigh-throughput screening of mutation libraries to develop PDbody.This integration is key to developing PDbody, a monobody product witha high affinity for PD-L1 and a preference for the acidic tumor microenvironment.Based on a single-domain monobody derived from human fibronectin,26−28 PDbody should have high stability with less immunogenicity. This evidence concerns the gene CD274 and neoplasm.