We discovered that CDK1 inhibition induced E-twenty-six specific sequence variant 2 (ETV2) to prevent the differentiation of EC-derived osteoblast-like cells and reduce arterial calcification in matrix Gla protein–deficient (Mgp–/–) mice, a model of vascular calcification (14–16, 26) and in diabetic Ins2Akita/+ mice, a model of vascular calcification in diabetes (15–18). Here, CDK1 is linked to calcification.