Then, we subjected these WTmCherry and KOmCherry mice to in vivo 45-minute myocardial ischemia, via coronary artery occlusion, followed by 4-day reperfusion, when cardiac macrophages were isolated for flow cytometry analysis of efferocytosis, with the gating strategy shown in Figure 3B. We observed that in I/R-injured KOmCherry hearts, macrophages (CD45+CD11b+F4/80+Ly6G–) engulfed a lower number of red mCherry-positive CMs than WTmCherry macrophages did (Figure 3, C and D). This evidence concerns the gene ITGAM and myocardial ischemia.