In addition to these pathways, we identified the top 10 hub genes by network-based analysis, including STAT3, FOS, TLR4, CPLX1, CPLX2, STX1B, VAMP1, MAPK3, and VEGFA. Leveraging the HumanNet-FN tool, we also identified top guide and candidate genes within the created networks based on overlapping putative targets mRNA identified for the serum miRNA profile and genes related to adult glioblastoma by the DisGenNET databases. This evidence concerns the gene TLR4 and glioblastoma.