Importantly, the methylation levels at these three CpG sites were negatively correlated with DIAPH3 expression (Padj=0.008, 0.002, and 0.003 for CpG 6, CpG 28 and CpG 29, respectively; Figure 4E), suggesting that the methylation of these sites contributes to DIAPH3 downregulation in glioblastoma samples. Here, DIAPH3 is linked to glioblastoma.