LRIG1 and urinary bladder carcinoma: Using a luciferase reporter assay, we confirmed this interaction and found that co-transfection of the wild-type LRIG1 with miR-214-3p mimics in bladder carcinoma cell lines (UMUC3 and T24) reduced the luciferase reporter activity, but not the mutant LRIG1 (Fig. 6E).