Further, to validate such findings in vivo, we subcutaneously inoculated the empty vector control (CTRL), FAM83D WT-overexpressed (FAM83D WT), and FAM83D M2-overexpressed (FAM83D M2) MCF7 cells in the athymic nude mice, and routinely monitored the tumor growth. This evidence concerns the gene SACK1D and neoplasm.