Therefore, we again performed IF staining of tumor sections to examine the expression of leiomyosarcoma markers, including vimentin, α–smooth muscle actin (α-SMA), caldesmon, and desmin, at the xenograft site, with the detected signals supporting these as leiomyosarcoma (Figure 1G and Supplemental Figure 4B). The gene discussed is DES; the disease is neoplasm.