The first study to show the FUS protein localizing to the presynaptic terminal in mammalian cells came in 2016 when STORM (stochastic optical reconstruction microscopy) super-resolution imaging verified its presence in cultured hippocampal neurons.175 This observation that FUS is physically present at the presynaptic terminal opened new possibilities for the fundamental role of FUS and the possible molecular mechanisms by which mutations in the protein lead to neurodegenerative disease. This evidence concerns the gene FUS and neurodegenerative disease.