As shown in Figure 2A, we have identified seven tsRNA fragments derived from tRNA-His-GUG or tRNA-His-GTG gene in the sncRNA-seq datasets of colon adenocarcinomas, there are two 3’-tRFs fragments (tRFdb-3013a and tRFdb-3013b) that can re-map to the cleavages of 3′ end of several different mature tRNA-His-GUG isoforms (Supplementary Figure 2A), and five tRF-1 fragments (ts-30, ts-43, ts-44, ts-1 and ts-88) that can re-map to the down-stream regions (35 bases) of primary tRNA-His-GTG genes. Here, TERF1 is linked to colon adenocarcinoma.