Having observed that NUP98 levels were lowered upon HIV-1 NL4.3 infection, we repeated the experiment to check the protein levels of all the NUPs under study (NUP98, NUP62, NUP155, NUP133, NUP107, and NUP85) in HEK293T cells upon proviral DNA pNL4.3 transfection, which allows the synthesis of the viral genomic RNA and proteins and the release of virions, mimicking the late stages of HIV-1 infection. Here, NUP85 is linked to HIV-1 infection.