Compared to the umbilical cords sample from the mother without GDM, PLAUR and SLIT2 were down-regulated in the GDM samples, and naive B cell, as well as T cells follicular helper, were upregulated, which suggested PLAUR may be a promoting factor for B cell naive, while SLIT2 may be a negative factor for T cells follicular helper in GDM samples. This evidence concerns the gene PLAUR and gestational diabetes.