We detected genes upregulated in PBT030 cells, such as SOX2, MYCN, NOS2, RUNX2, and VEGFA, while PBT147 cells upregulated level of PTEN, STAT6, CA9 and TLR2, demonstrating differences among PBT cell lines, which can be explained by various driving mutations and heterogeneity in glioma lines (Figures 3A–D and Supplementary Figures 2A, B). The gene discussed is NOS2; the disease is glioma.