Genotyping of HLA class I (HLA-A/B/C) and class II (HLA-DRB1/3/4/5 and HLA-DQB1/DPB1/DPA1/DQA1) genes was performed using NGS to establish possible connections with CLL. This evidence concerns the gene HLA-DRB1 and B-cell chronic lymphocytic leukemia.