PRKN and in situ carcinoma: In light of this, we treated A2780 and A2780 CIS cells with Bafilomycin A1, which inhibits the vacuolar type H+ -translocation ATPase (V-ATPase), and/or with CCCP, which induces (i) depolarization of mitochondria, (ii) PINK1 accumulation, (iii) recruitment of Parkin and (iv) mitophagy concretization.