In addition, the results of chemical genetics indicated that inhibiting PVN<sup>CRH</sup> neurons has a similar protective effect on MIRI as EA pretreatment, and the activation of PVN<sup>CRH</sup> neurons can counteract this protective effect.<h4>Conclusion</h4>EA pretreatment can inhibit PVN<sup>CRH</sup> neurons and improve MIRI by inhibiting sympathetic nerve, which offers fresh perspectives on the application of acupuncture in the management of cardiovascular disease. Here, CRH is linked to cardiovascular disorder.