Consistent with these data, expression of WT ATP6AP1 but not the two mutants in ATP6AP1-knockdown cells could restore proliferation (Fig. 7c), anchorage-independent growth (Fig. 7d, e), and migration (Fig. 7f, g; Supplementary information, Fig. S8d, e), providing further evidence that the Rheb GEF activity of ATP6AP1 is critical for cancer cell proliferation and migration. This evidence concerns the gene ATP6AP1 and cancer.