mIF on FFPE samples with antibodies of CD4, CD8α, FOXP3, Granzyme B, and pan CK (Fig. 5a and Supplementary Fig. 5) were applied to analyze the tumor regions of pre- and post-ipilimumab treatment for 4 patients (P6, P7, P4 and P8). This evidence concerns the gene GZMB and neoplasm.