PD-L1 expression is detected by PD-L1 immunohistochemical assay and quantified by tumor proportion score (TPS), which is defined by the percentage of stained tumor cells over total tumor cells.24 PD-L1 TPS of pre-treatment tumor tissues in CR patients was significantly higher than that in PD patients (Fig. 3a), while PD-1 expression on TIL correlated with poor response to treatment (Fig. 3b). The gene discussed is CD274; the disease is neoplasm.