In our time-course analyses in PEDV-infected or N-expressing cells, p53 did increase at the early time point (12 h after PEDV infection or N expression) but markedly reduced at 24 h after virus infection or from 24 to 48 h after transfection (Figs. 1, A–C, 2A and 3B). This evidence concerns the gene TP53 and viral infectious disease.