G3BP1 and osteosarcoma: We used the formation of stress granules (SGs) in human osteosarcoma cells (U2OS) expressing EGFP‐G3BP1 as the experimental model for studying intracellular LLPS.[12] To measure the intracellular diffusion dynamics, we loaded quantum dots (QDs) into the cell cytosol through the osmotic lysis of pinocytic vesicles.