We next investigated how this pharynx→NJP→NTS circuit functions in pharyngeal inflammation–induced anxiety-like behaviors by injecting retro-hSyn-FlpO into the pharynx, a Cre and Flp-dependent AAV carrying eNpHR-enhanced yellow fluorescent protein (AAV-hSyn-Con Fon-eNpHR-EYFP) into the bilateral NJP ganglia, and retro-AAV-hSyn-Cre-mCherry into the bilateral NTS. The gene discussed is RIC8B; the disease is Anxiety.