We, therefore, quantitatively measured the eye size and showed a significantly smaller eye area upon Prdm15 depletion describing a strong microphthalmia phenotype (Figures 3B, C) and a coloboma phenotype (Figures 3D, E), consistent with the ocular defects in patients with HPE or GAMOS (Roessler and Muenke, 1998; Al-Rakan et al., 2018; Lin et al., 2018; Racine and Golden, 2021; Ramakrishnan and Gupta, 2023). This evidence concerns the gene PRDM15 and holoprosencephaly.