Comparing the two PRDM15 mutation variants, the embryos rescued with hPRDM15-C844Y RNA tended to have smaller eyes and more coloboma than the embryos rescued with the hPRDM15-M154K variant (Figures 3B–E), supporting the C884Y variant as more pathogenic in the anterior neural development. The gene discussed is PRDM15; the disease is coloboma.