And the factors, including pTau217+ EVs (p < 0.0001), the distribution of GABRD+ carrying pTau217 EVs (p < 0.0001), the distribution of GPR162+ carrying pTau217 EVs (p < 0.0001), GABRD+ carrying pTau217 EVs (p < 0.05) and GABRD+ EVs (p < 0.05) were significantly associated with the differential diagnosis of AD vs NAD. Here, GPR162 is linked to Alzheimer disease.