Regarding AD vs NAD in discovery cohort, the AUC was 0.5079 for GABRD+-carrying pTau217 EVs, 0.7309 for GPR162+-carrying pTau217 EVs, 0.7422 for the distribution of GABRD+ carrying pTau217 EVs, and 0.6756 for the distribution of GPR162+ carrying pTau217 EVs. The gene discussed is GPR162; the disease is Alzheimer disease.