The findings revealed that, in comparing AD with HC in discovery cohort, the AUC was 0.9166 for GABRD+-carrying pTau217 EVs, 0.8183 for GPR162+-carrying pTau217 EVs, 0.5149 for the distribution of GABRD+ carrying pTau217 EVs, and 0.5211 for the distribution of GPR162+ carrying pTau217 EVs. Here, GPR162 is linked to Alzheimer disease.