ATXN3 and Machado-Joseph disease: Blinded manual counting of the ataxin-3 aggregates within the pons and medulla oblongata revealed more ataxin-3-positive aggregates in the brains of MJD mice compared to WT controls, confirmed by the finding of a significant effect of genotype on the number of aggregates present (Fig. 5B, C; p = 0.0001 for each).