GUCY2C and malignant colon neoplasm: For example, Wu et al. found that the combination of plasma 5’-TRF-Gly-GCC with carcinoembryonic antigen and carbohydrate antigen 199 in colon cancer patients increased the AUC value to 0.926 [28]; Importantly, Panoutsopoulou et al. found a significant correlation between i-tRF-GlyGCC and early progression as well as poor overall survival [29]; In addition, Li et al. discovered that the expression level of serum tRF-29-R9J8909NF5JP is significantly elevated in gastric cancer tissues, and its high expression is associated with lower survival rates [30].