The newly synthesized compounds 5a-f, 9a-c and 10a-c were screened for their in vitro anti-proliferative activity via standard MTT assay36,37, using three human tumor cell lines namely; colorectal carcinoma HCT-116, hepatocellular carcinoma HEPG-2 and breast cancer MCF-7 cell lines in which EGFR is expressed27. Here, EGFR is linked to breast carcinoma.