The liver tissues exhibited abnormal morphology and the presence of fibrosis, as evidenced by hematoxylin and eosin (H&E) staining and Masson’s trichrome staining (Fig. 1F, G) Significantly, the upregulation of IGF2BP2 in fibrotic livers was validated through quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis, and demonstrated a parallel increase in the expression of key genes (ACTA2, COL1α1) associated with the advancement of liver fibrosis (Fig. 1H, I). This evidence concerns the gene COL1A1 and Hepatic fibrosis.