Further studies on CT2A and GL261 cells treated with stiripentol (Fig. 4d and Supplementary Fig. S7b) or expressing shLdha (Fig. 4e, f) demonstrated that LDHA inhibition downregulated the expression of Hoxa9 andYap1, but had no effect on Eed and Ezh2. Next, we aimed to confirm whether YAP1, JNK/STAT, AKT/mTOR, Raf/ERK, and STK33 pathways are regulated by LDHA in glioblastoma cells. Here, EED is linked to glioblastoma.