Based on our coronavirus pseudovirus (PsV) inhibition assay,44,45 we found that 14a potently and broadly inhibited pseudotyped SARS-CoV-2, SARS-CoV, MERS-CoV, and HCoV-229E infection with EC50 values ranging from 3.33 nM to 29.75 nM (293 T/ACE2 cells for SARS-CoV-2 PsV and Huh-7 cells for other PsVs). This evidence concerns the gene ACE2 and infection.