NUSAP1 and cancer: We knockdown NUSAP1 using three siRNAs, siNUSAP1-1 didn’t show any effect (Fig. 9C, D), thus we continue the study using the siNUSAP1-2 and siNUSAP1-3 site, the proliferation ability of HepG2.2.15 was significantly reduced (Fig. 9E), as well as a decrease in colony number was observed in the NUSAP1 knockdown cells (Supplementary Fig. 3), suggesting that NUSAP1 may promote cancer cell proliferation.