CYCS and neoplasm: Figure 4E–I revealed that the combination therapy strikingly reduced the levels of associated proteins, indicating its potential to suppress tumor cell proliferation. Subsequently, the cell apoptosis in tumor tissues was evaluated, and the expression of pro-apoptotic proteins (c-caspase3, Bax, and Cytochrome C) was observed to be significantly upregulated in the tumor tissues of mice treated with radiotherapy plus nifuroxazide, whereas the expression of anti-apoptotic protein (BCL-2) was downregulated (Figure 4J–N).