Preliminary results showed that compound 199 possesses the strongest inhibitory activity, among the tested compounds, on the proliferation of fibroid synovial cells (RA-FLSs, IC50 = 1.78 μM) compared to the reference methotrexate (IC50 = 5.0 μM), and it also has inhibitory effect on RA related cytokines IL-1, IL-6, and TNF-α (Figure 4). This evidence concerns the gene IL1B and rheumatoid arthritis.