Other classical example of gene, which is mutated at later stages, is NPM1. In DNTM3a and NPM1 mutated AML, both NPM1 and DNMT3a mutations were present only in CD45dimCD33+AML blasts, whereas HSCs/MPPs, lineage-committed progenitors, and CD33−mature cells harbored only DNTM3a mutation (57). The gene discussed is DNMT3A; the disease is acute myeloid leukemia.