SASH3 and infection: To evaluate the role of SadP and Sly in the translocation of zoonotic SS2CC1 S. suis across the enteroid monolayer, we constructed ΔsadP, Δsly, and ΔsadPΔsly mutants in the strain 05ZYH33. We assessed the translocation frequency of 05ZYH33 WT and KO mutants in the same nine monolayer infection experiments used to study the role of Gb3-positive cells in S. suis translocation described above.